#BIOPROFILE 400 MANUAL MANUAL#
These results suggest that the system has the potential to dramatically reduce the manual labor involved in monitoring mammalian cell bioprocesses without altering the quality of the data obtained, and integration with a bioreactor control system will allow feedback control of parameters previously available only for off-line monitoring. Other key parameters, however, are still typically monitored via manual sampling and several. In general, BioProfile FLEX measurements were equivalent to those obtained using reference analyzers, and the auto-sampler did not alter the samples it provided to the BioProfile FLEX. Evaluation of the BioProfile FLEX was conducted in two separate laboratories and involved two BioProfile FLEX analyzers and two sets of reference analyzers (Nova BioProfile 400, Beckman-Coulter Vi-Cell AS, and Advanced Instruments Osmometer 3900), 13 CHO cell lines and over 20 operators. This is the first report on the development and function of this integrated analyzer and an auto-sampler prototype for monitoring of mammalian cell cultures. In addition, a novel automated sampling system has also been developed that allows the BioProfile FLEX to automatically analyze the culture conditions in as many as ten bioreactors. bioprofile flex test menu cd cv gln glu nh 4 + gluc lac ph po 2 pco 2 na+ k+ ca+ +. If you need further assistance, please call VWR Customer Service at 1-80. Alternatives may be available by searching with the VWR Catalog Number listed above. 5 minutes with just 265 microliters of sample from 96- well plates, the built- in 24- position tray, or via manual sampling. Sealing Washer Kit (pH) For BioProfile 200 / 250 / 400.
An integrated multi-functional off-line analyzer, the BioProfile FLEX (NOVA Biomedical, Waltham MA) has been developed, which combines the functionality of three off-line analyzers (a cell counter, an osmometer, and a gas/electrolyte & nutrient/metabolite bio-profile analyzer) into one device. nova medical bioprofile 400 analyzer with autosampler, s/ n: t0310, model: 30706, v, 0. Off-line monitoring of such cultivations via manual sampling is often labor-intensive and can introduce operator-dependent error into the process. Nutrient and metabolite concentrations were analyzed Nova Bioprofile 400. Mammalian cell-based bioprocesses are used extensively for production of therapeutic proteins. We feed both experimental groups by daily manual replacement of media over the.
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